Advances in tissue engineering require the development of new biomaterials with adequate properties of cell attachment and growth. The properties of biomaterials can be improved by incorporation of bioactive molecules to enhance in vitro and/or in vivo functions. We study the role of a wheat germin-like protease inhibitor (GLPI), free or immobilized in biocompatible matrices to improve cell-attachment ability on different mammalian cell lines. The phylogenetic relationships and functional diversity of the GLPI were analyzed among diverse genera to get insights into sequence motif conservations. The cytocompatibility effect of free GLPI on C2C12 premyoblastic cells and B16 cells as tumoral model has been tested. GLPI promoted proliferation and metabolic activity of both cell types on in vitro models, not showing cytotoxic effects. Furthermore, GLPI was immobilized in chitin microparticles and in chitosan films; we demonstrated an accelerated cell adhesion process in both biomaterials.
The quest for improved biomaterials is driven by the growing demands of tissue engineering. Our research delves into this realm by exploring the potential of incorporating bioactive molecules to enhance the functionalities of these materials. By focusing on the wheat germin-like protease inhibitor (GLPI), we aim to elucidate its role in improving cell attachment and growth across various cell lines.
Through a comprehensive analysis, we investigate the phylogenetic relationships and functional diversity of GLPI among different genera. This approach offers valuable insights into sequence motif conservations, providing a foundation for understanding the molecular mechanisms underlying its bioactivity. Such understanding is crucial for informed design and optimization of biomaterials for tissue engineering applications.